|Application ||WB, E|
|Other Accession||Q9WV69, Q08DM1, NP_001107609.1, NP_001107608.1|
|Calculated MW||45514 Da|
|Antigen Region||249-277 aa|
|Other Names||Dematin, Dematin actin-binding protein, Erythrocyte membrane protein band 49, DMTN, DMT, EPB49|
|Target/Specificity||This EPB49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human EPB49.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EPB49 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Membrane-cytoskeleton-associated protein with F-actin- binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Plays also a role as a modulator of actin dynamics in fibroblasts; acts as a negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation.|
|Cellular Location||Cytoplasm. Cytoplasm, cytosol. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Cell membrane. Membrane. Endomembrane system. Cell projection. Note=Localized at the spectrin-actin junction of erythrocyte plasma membrane. Localized to intracellular membranes and the cytoskeletal network. Localized at intracellular membrane-bounded organelle compartment in platelets that likely represent the dense tubular network membrane. Detected at the cell membrane and at the parasitophorous vacuole in malaria-infected erythrocytes at late stages of plasmodium berghei or falciparum development|
|Tissue Location||Expressed in platelets (at protein level). Expressed in heart, brain, lung, skeletal muscle, and kidney|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Dematin, or EPB49, is an actin-bundling protein originally identified in the erythroid membrane skeleton. Its actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase and is restored after dephosphorylation (Rana et al., 1993 [PubMed 8341682]).
Vugmeyster, L., et al. J. Biomol. NMR 47(2):155-162(2010)
Chen, L., et al. Protein Sci. 18(3):629-636(2009)
Vugmeyster, L., et al. J. Biomol. NMR 43(1):39-50(2009)
Vermeulen, W., et al. J. Mol. Biol. 359(5):1277-1292(2006)
Frank, B.S., et al. J. Biol. Chem. 279(9):7909-7916(2004)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.