|Application ||WB, E|
|Other Accession||Q9WV69, Q08DM1, NP_001107609.1, NP_001107608.1|
|Calculated MW||45514 Da|
|Antigen Region||249-277 aa|
|Other Names||Dematin, Dematin actin-binding protein, Erythrocyte membrane protein band 49, DMTN, DMT, EPB49|
|Target/Specificity||This EPB49 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 249-277 amino acids from the Central region of human EPB49.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||EPB49 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Membrane-cytoskeleton-associated protein with F-actin- binding activity that induces F-actin bundles formation and stabilization. Its F-actin-bundling activity is reversibly regulated upon its phosphorylation by the cAMP-dependent protein kinase A (PKA). Binds to the erythrocyte membrane glucose transporter-1 SLC2A1/GLUT1, and hence stabilizes and attaches the spectrin-actin network to the erythrocytic plasma membrane. Plays a role in maintaining the functional integrity of PKA-activated erythrocyte shape and the membrane mechanical properties. Plays also a role as a modulator of actin dynamics in fibroblasts; acts as a negative regulator of the RhoA activation pathway. In platelets, functions as a regulator of internal calcium mobilization across the dense tubular system that affects platelet granule secretion pathways and aggregation. Also required for the formation of a diverse set of cell protrusions, such as filopodia and lamellipodia, necessary for platelet cell spreading, motility and migration. Acts as a tumor suppressor and inhibits malignant cell transformation.|
|Cellular Location||Cytoplasm. Cytoplasm, cytosol. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Cell membrane. Membrane. Endomembrane system. Cell projection. Note=Localized at the spectrin-actin junction of erythrocyte plasma membrane. Localized to intracellular membranes and the cytoskeletal network. Localized at intracellular membrane-bounded organelle compartment in platelets that likely represent the dense tubular network membrane. Detected at the cell membrane and at the parasitophorous vacuole in malaria-infected erythrocytes at late stages of plasmodium berghei or falciparum development|
|Tissue Location||Expressed in platelets (at protein level). Expressed in heart, brain, lung, skeletal muscle, and kidney|
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Provided below are standard protocols that you may find useful for product applications.
Dematin, or EPB49, is an actin-bundling protein originally identified in the erythroid membrane skeleton. Its actin-bundling activity is abolished upon phosphorylation by cAMP-dependent protein kinase and is restored after dephosphorylation (Rana et al., 1993 [PubMed 8341682]).
Vugmeyster, L., et al. J. Biomol. NMR 47(2):155-162(2010)
Chen, L., et al. Protein Sci. 18(3):629-636(2009)
Vugmeyster, L., et al. J. Biomol. NMR 43(1):39-50(2009)
Vermeulen, W., et al. J. Mol. Biol. 359(5):1277-1292(2006)
Frank, B.S., et al. J. Biol. Chem. 279(9):7909-7916(2004)
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