|Application ||WB, E|
|Calculated MW||23625 Da|
|Antigen Region||178-206 aa|
|Other Names||Rho-related GTP-binding protein RhoF, Rho family GTPase Rif, Rho in filopodia, RHOF, ARHF, RIF|
|Target/Specificity||This RHOF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-206 amino acids from the C-terminal region of human RHOF.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RHOF Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin- based morphology.|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytoskeleton|
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Provided below are standard protocols that you may find useful for product applications.
Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Causes the formation of thin, actin-rich surface projections called filopodia. Functions cooperatively with CDC42 and Rac to generate additional structures, increasing the diversity of actin-based morphology.
Fan, L., et al. J. Cell. Sci. 123 (PT 8), 1247-1252 (2010) :
Carbone, A., et al. Genes Chromosomes Cancer 47(12):1067-1075(2008)
Ellis, S., et al. Curr. Biol. 10(21):1387-1390(2000)
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