|Application ||WB, E|
|Calculated MW||127062 Da|
|Antigen Region||228-257 aa|
|Other Names||Unconventional myosin-Ie, Myosin-Ic, Unconventional myosin 1E, MYO1E, MYO1C|
|Target/Specificity||This MYO1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-257 amino acids from the N-terminal region of human MYO1E.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MYO1E Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which are then moved relative to actin filaments. Binds to membranes containing anionic phospholipids via its tail domain. Required for normal morphology of the glomerular basement membrane, normal development of foot processes by kidney podocytes and normal kidney function. In dendritic cells, may control the movement of class II-containing cytoplasmic vesicles along the actin cytoskeleton by connecting them with the actin network via ARL14EP and ARL14.|
|Cellular Location||Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasmic vesicle. Cytoplasmic vesicle, clathrin-coated vesicle Cell junction. Note=Colocalizes with F-actin (By similarity). In cultured podocytes, it localizes close to and is associated with the cytoplasmic membrane, with enrichment at the lamellipodia tips. Colocalizes with cytoplasmic vesicles, including endocytic clathrin-coated vesicles. Colocalizes with dynamin at cytoplasmic vesicles.|
|Tissue Location||Expressed in the immune system. In the kidney, predominantly expressed in the glomerulus, including podocytes|
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Provided below are standard protocols that you may find useful for product applications.
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments (By similarity).
Feeser, E.A., et al. Biochemistry 49(43):9353-9360(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Krendel, M., et al. FEBS Lett. 581(4):644-650(2007)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
El Mezgueldi, M., et al. J. Biol. Chem. 277(24):21514-21521(2002)
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