|Application ||WB, E|
|Other Accession||Q91V17, NP_115644.1|
|Calculated MW||23783 Da|
|Antigen Region||59-86 aa|
|Other Names||E3 ubiquitin-protein ligase ZNRF1, 632-, Nerve injury-induced gene 283 protein, Zinc/RING finger protein 1, ZNRF1, NIN283|
|Target/Specificity||This ZNRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from the Central region of human ZNRF1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZNRF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||E3 ubiquitin-protein ligase that mediates the ubiquitination of AKT1 and GLUL, thereby playing a role in neuron cells differentiation. Plays a role in the establishment and maintenance of neuronal transmission and plasticity. Regulates Schwann cells differentiation by mediating ubiquitination of GLUL. Promotes neurodegeneration by mediating 'Lys-48'-linked polyubiquitination and subsequent degradation of AKT1 in axons: degradation of AKT1 prevents AKT1-mediated phosphorylation of GSK3B, leading to GSK3B activation and phosphorylation of DPYSL2/CRMP2 followed by destabilization of microtubule assembly in axons (Probable).|
|Cellular Location||Endosome. Lysosome. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Peripheral membrane protein. Note=Associated with synaptic vesicle membranes in neurons|
|Tissue Location||Expressed primarily in the nervous system, with expression higher in developing brain relative to adult Expressed at low levels in testis and thymus|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
In a study identifying genes in rat that are upregulated in response to nerve damage, a gene which is highly expressed in ganglia and in the central nervous system was found. The protein encoded by the rat gene contains both a zinc finger and a RING finger motif and is localized in the endosome/lysosome compartment, indicating that it may be involved in ubiquitin-mediated protein modification. The protein encoded by this human gene is highly similar in sequence to that encoded by the rat gene. [provided by RefSeq].
Dastani, Z., et al. Eur. J. Hum. Genet. 18(3):342-347(2010)
Yoshida, K., et al. Biochem. Biophys. Res. Commun. 389(3):506-511(2009)
Markson, G., et al. Genome Res. 19(10):1905-1911(2009)
van Wijk, S.J., et al. Mol. Syst. Biol. 5, 295 (2009) :
Araki, T., et al. J. Neurosci. 23(28):9385-9394(2003)
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