|Application ||WB, E|
|Other Accession||P56222, P31360, NP_005595.2|
|Calculated MW||46893 Da|
|Antigen Region||199-226 aa|
|Other Names||POU domain, class 3, transcription factor 2, Brain-specific homeobox/POU domain protein 2, Brain-2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Oct-7, Octamer-binding transcription factor 7, OTF-7, POU3F2, BRN2, OCT7, OTF7|
|Target/Specificity||This POU3F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-226 amino acids from the Central region of human POU3F2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POU3F2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BRN2, OCT7, OTF7|
|Function||Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).|
|Tissue Location||Expressed specifically in the neuroectodermal cell lineage|
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Provided below are standard protocols that you may find useful for product applications.
POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene [removed]Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).
Kobi, D., et al. Pigment Cell Melanoma Res 23(3):404-418(2010)
Flammiger, A., et al. J. Invest. Dermatol. 129(4):945-953(2009)
Cabos-Siguier, B., et al. Protein Expr. Purif. 64(1):39-46(2009)
Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009) :
Potkin, S.G., et al. Schizophr Bull 35(1):96-108(2009)
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