|Application ||WB, E|
|Calculated MW||42119 Da|
|Antigen Region||277-306 aa|
|Other Names||CST complex subunit STN1, Oligonucleotide/oligosaccharide-binding fold-containing protein 1, Suppressor of cdc thirteen homolog, OBFC1, STN1|
|Target/Specificity||This OBFC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the C-terminal region of human OBFC1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||OBFC1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites.|
|Cellular Location||Nucleus. Chromosome, telomere.|
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Provided below are standard protocols that you may find useful for product applications.
OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).
Levy, D., et al. Proc. Natl. Acad. Sci. U.S.A. 107(20):9293-9298(2010)
Miyake, Y., et al. Mol. Cell 36(2):193-206(2009)
Wan, M., et al. J. Biol. Chem. 284(39):26725-26731(2009)
Casteel, D.E., et al. J. Biol. Chem. 284(9):5807-5818(2009)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
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