|Application ||WB, E|
|Calculated MW||27798 Da|
|Antigen Region||71-98 aa|
|Other Names||Pleckstrin homology domain-containing family F member 2, PH domain-containing family F member 2, Endoplasmic reticulum-associated apoptosis-involved protein containing PH and FYVE domains, EAPF, PH and FYVE domain-containing protein 2, Phafin-2, Phafin2, Zinc finger FYVE domain-containing protein 18, PLEKHF2, ZFYVE18|
|Target/Specificity||This PLEKHF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-98 amino acids from the Central region of human PLEKHF2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PLEKHF2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in early endosome fusion upstream of RAB5, hence regulating receptor trafficking and fluid-phase transport. Enhances cellular sensitivity to TNF-induced apoptosis (PubMed:18288467).|
|Cellular Location||Early endosome membrane; Peripheral membrane protein. Endoplasmic reticulum. Note=Colocalizes with EEA1 and RAB5 at endosomal membrane fusion hot spots (PubMed:19995552). May translocate to the endoplasmic reticulum in the early phase of apoptosis (PubMed:18288467).|
|Tissue Location||Expressed in placenta, ovary and small intestine, as well as in heart and pancreas. Also expressed in peripheral blood mononuclear cells and dendritic cells|
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Provided below are standard protocols that you may find useful for product applications.
PLEKHF2 (Phafin 2) is a PH and FYVE domain containing protein 2. The FYVE domain and Zinc binding domain targets proteins to membrane lipids via interaction with phosphatidylinositol 3 phosphate, PI3P. The FYVE domain and Zinc binding domain present in Fab1, YOTB, Vac1, and EEA1.
Lin, W.J., et al. Biochem. Biophys. Res. Commun. 391(1):1043-1048(2010)
Li, C., et al. J. Mol. Med. 86(4):471-484(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
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