|Application ||WB, E|
|Other Accession||Q8BJQ2, Q29RP1, NP_001017416.1, NP_001017415.1|
|Calculated MW||88207 Da|
|Antigen Region||662-690 aa|
|Other Names||Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1, hUBP, Ubiquitin thioesterase 1, Ubiquitin-specific-processing protease 1, USP1|
|Target/Specificity||This USP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 662-690 amino acids from the C-terminal region of human USP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||USP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2 (PubMed:15694335). Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA (PubMed:16531995). Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity (PubMed:18082604, PubMed:26388029).|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. The protein specifically deubiquitinates a protein in the Fanconi anemia (FA) DNA repair pathway. Alternate transcriptional splice variants have been characterized.
Barroso, E., et al. Breast Cancer Res. Treat. 118(3):655-660(2009)
McWilliams, R.R., et al. Cancer Epidemiol. Biomarkers Prev. 18(9):2549-2552(2009)
Cohn, M.A., et al. J. Biol. Chem. 284(8):5343-5351(2009)
Cohn, M.A., et al. Mol. Cell 28(5):786-797(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
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