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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   CUL2 Antibody (N-term)   

CUL2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - CUL2 Antibody (N-term) AP16900a
    CUL2 Antibody (N-term) (Cat. #AP16900a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the CUL2 antibody detected the CUL2 protein (arrow).
  • WB - CUL2 Antibody (N-term) AP16900a
    CUL2 Antibody (N-term) (Cat. #AP16900a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the CUL2 antibody detected the CUL2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q13617
Other Accession Q9D4H8, NP_003582.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 86983 Da
Antigen Region 150-179 aa
Additional Information
Gene ID 8453
Other Names Cullin-2, CUL-2, CUL2
Target/Specificity This CUL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the N-terminal region of human CUL2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCUL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CUL2
Function Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (PubMed:27565346). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).
Research Areas
Citations (0)

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Background

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

References

Park, S.W., et al. APMIS 117(12):880-885(2009)
Argyropoulos, G., et al. Physiol. Genomics 36(2):79-88(2009)
Barrett, J.C., et al. Nat. Genet. 40(8):955-962(2008)
Maeda, Y., et al. J. Biol. Chem. 283(23):16084-16092(2008)
Huh, K., et al. J. Virol. 81(18):9737-9747(2007)

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$ 295.00
$ 99.00
Cat# AP16900a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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