|Application ||WB, E|
|Calculated MW||37687 Da|
|Antigen Region||162-191 aa|
|Other Names||Dual specificity protein phosphatase 12, Dual specificity tyrosine phosphatase YVH1, DUSP12|
|Target/Specificity||This DUSP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 amino acids from the Central region of human DUSP12.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DUSP12 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Dual specificity phosphatase; can dephosphorylate both phosphotyrosine and phosphoserine or phosphothreonine residues. Can dephosphorylate glucokinase (in vitro) (By similarity). Has phosphatase activity with the synthetic substrate 6,8-difluoro-4- methylumbelliferyl phosphate and other in vitro substrates (PubMed:10446167, PubMed:24531476).|
|Cellular Location||Nucleus. Cytoplasm, cytosol. Note=Primarily nuclear. Detected in a mesh-like pattern in the cytosol|
|Tissue Location||Ubiquitous, highest expression in spleen, testis, ovary, and peripheral blood leukocytes and lower expression in liver and lung|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product is the human ortholog of the Saccharomyces cerevisiae YVH1 protein tyrosine phosphatase. It is localized predominantly in the nucleus, and is novel in that it contains, and is regulated by a zinc finger domain.
Biernacki, M.A., et al. Cancer Res. 70(3):906-915(2010)
Bonham, C.A., et al. J. Biol. Chem. 284(34):22853-22864(2009)
Sharda, P.R., et al. Biochem. J. 418(2):391-401(2009)
Gao, F., et al. Zhonghua Yi Xue Za Zhi 88(32):2250-2253(2008)
Hasstedt, S.J., et al. Ann. Hum. Genet. 72 (PT 2), 163-169 (2008) :
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