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PTTG2 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - PTTG2 Antibody (Center) AP16911C
    Anti-PTTG2 Antibody (Center) at 1:2000 dilution + human liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PTTG2 Antibody (Center) AP16911C
    PTTG2 Antibody (Center) (Cat. #AP16911c) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the PTTG2 antibody detected the PTTG2 protein (arrow).
  • IF - PTTG2 Antibody (Center) AP16911C
    Fluorescent confocal image of MCF-7 cell stained with PTTG2 Antibody (Center)(Cat#AP16911c).MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PTTG2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). PTTG2 immunoreactivity is localized to Cytoplasm significantly.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9NZH5
Other Accession NP_006598.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22302 Da
Antigen Region 56-84 aa
Additional Information
Gene ID 10744
Other Names Securin-2, Pituitary tumor-transforming gene 2 protein, PTTG2
Target/Specificity This PTTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-84 amino acids from the Central region of human PTTG2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPTTG2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PTTG2
Cellular Location Cytoplasm. Nucleus
Tissue Location Expressed at low levels in the pituitary, liver, spleen, prostate, testis, ovary, small intestine and colon Also expressed in various pituitary, testicular, liver and ovarian tumors.
Research Areas
Citations ( 0 )


Chen, L., et al. Gene 248 (1-2), 41-50 (2000) :
Prezant, T.R., et al. J. Clin. Endocrinol. Metab. 84(3):1149-1152(1999)

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$ 295.00
$ 99.00
Cat# AP16911C
(40 western blots)
Availability: In Stock
Bulk Size
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