|Application ||WB, E|
|Other Accession||P62257, Q32LN1, NP_874356.1, NP_003335.1|
|Calculated MW||20655 Da|
|Antigen Region||42-70 aa|
|Other Names||Ubiquitin-conjugating enzyme E2 H, UbcH2, Ubiquitin carrier protein H, Ubiquitin-conjugating enzyme E2-20K, Ubiquitin-protein ligase H, UBE2H|
|Target/Specificity||This UBE2H antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-70 amino acids from the Central region of human UBE2H.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||UBE2H Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'- and 'Lys-48'-linked polyubiquitination. Capable, in vitro, to ubiquitinate histone H2A.|
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Provided below are standard protocols that you may find useful for product applications.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs. Two alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.
Lausen, J., et al. J. Biol. Chem. 285(8):5338-5346(2010)
Martin, I., et al. Amyotroph Lateral Scler 10 (5-6), 432-435 (2009) :
de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009)
Martin, I., et al. Amyotroph Lateral Scler, 1-4 (2008) In press :
Kim, H.J., et al. Immunol. Lett. 95(2):155-159(2004)
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