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PI4K2A Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PI4K2A Antibody (C-term) AP16933b
    All lanes : Anti-PI4K2A Antibody (C-term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PI4K2A Antibody (C-term) AP16933b
    PI4K2A Antibody (C-term) (Cat. #AP16933b) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the PI4K2A antibody detected the PI4K2A protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BTU6
Other Accession Q99M64, Q6PE18, NP_060895.1
Reactivity Human
Predicted Zebrafish, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54022 Da
Antigen Region 387-415 aa
Additional Information
Gene ID 55361
Other Names Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI4K2A
Target/Specificity This PI4K2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of human PI4K2A.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI4K2A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PI4K2A
Function Membrane-bound phosphatidylinositol-4 kinase (PI4- kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).
Cellular Location Golgi apparatus, trans-Golgi network membrane; Lipid-anchor. Membrane raft. Cell projection, dendrite {ECO:0000250|UniProtKB:Q2TBE6}. Cell junction, synapse, presynaptic cell membrane {ECO:0000250|UniProtKB:Q2TBE6}. Cell junction, synapse, synaptosome {ECO:0000250|UniProtKB:Q2TBE6} Mitochondrion {ECO:0000250|UniProtKB:Q2TBE6}. Endosome Cytoplasmic vesicle. Membrane; Lipid-anchor. Cell membrane Note=Localized in neuronal cell body. Transported from neuronal cell body to neuron projections in a BLOC-1- and AP-3-complexes- dependent manner. Enriched in neurite tips and neuron projections in a BLOC-1- and AP-3-complexes-dependent manner (By similarity) Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. {ECO:0000250|UniProtKB:Q2TBE6, ECO:0000250|UniProtKB:Q99M64}
Tissue Location Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.
Research Areas
Citations (0)

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Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs (Barylko et al., 2001 [PubMed 11244087]).


Li, J., et al. Oncogene 29(17):2550-2559(2010)
Qin, Y., et al. J. Biol. Chem. 284(34):22544-22548(2009)
Salazar, G., et al. J. Biol. Chem. 284(3):1790-1802(2009)
Craige, B., et al. Mol. Biol. Cell 19(4):1415-1426(2008)
Olsen, J.V., et al. Cell 127(3):635-648(2006)

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$ 295.00
$ 99.00
Cat# AP16933b
(40 western blots)
Availability: In Stock
Bulk Size
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