|Application ||WB, E|
|Calculated MW||73603 Da|
|Antigen Region||162-191 aa|
|Other Names||Nucleolar and coiled-body phosphoprotein 1, 140 kDa nucleolar phosphoprotein, Nopp140, Hepatitis C virus NS5A-transactivated protein 13, HCV NS5A-transactivated protein 13, Nucleolar 130 kDa protein, Nucleolar phosphoprotein p130, NOLC1, KIAA0035, NS5ATP13|
|Target/Specificity||This NOLC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 amino acids from the N-terminal region of human NOLC1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NOLC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).|
|Cellular Location||Nucleus, nucleolus. Cytoplasm. Note=Shuttles between the nucleolus and the cytoplasm. At telophase it begins to assemble into granular-like pre-nucleolar bodies which are subsequently relocated to nucleoli at the early G1-phase|
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Provided below are standard protocols that you may find useful for product applications.
Related to nucleologenesis, may play a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus. It has intrinsic GTPase and ATPase activities. May play an important role in transcription catalyzed by RNA polymerase I.
Thiry, M., et al. Histochem. Cell Biol. 132(2):129-140(2009)
Hwang, Y.C., et al. Am. J. Pathol. 175(1):342-354(2009)
Renvoise, B., et al. Hum. Mol. Genet. 18(7):1181-1189(2009)
Lee, W.K., et al. Biochem. Biophys. Res. Commun. 376(2):439-444(2008)
Tsai, Y.T., et al. J. Biomed. Sci. 15(4):471-486(2008)
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