|Application ||WB, E|
|Calculated MW||53962 Da|
|Antigen Region||171-198 aa|
|Other Names||DNA-directed RNA polymerase I subunit RPA49, RNA polymerase I subunit A49, DNA-directed RNA polymerase I subunit E, RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53, POLR1E, PAF53, PRAF1|
|Target/Specificity||This POLR1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-198 amino acids from the Central region of human POLR1E.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||POLR1E Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity).|
|Cellular Location||Nucleus, nucleolus|
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Provided below are standard protocols that you may find useful for product applications.
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity).
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006)
Yamamoto, K., et al. Mol. Cell. Biol. 24(14):6338-6349(2004)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
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