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POLR1E Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9GZS1 |
| Other Accession | NP_071935.1 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 47260 Da |
| Antigen Region | 171-198 aa |
| Gene ID | 64425 |
|---|---|
| Other Names | DNA-directed RNA polymerase I subunit RPA49, RNA polymerase I subunit A49, DNA-directed RNA polymerase I subunit E, RNA polymerase I-associated factor 1, RNA polymerase I-associated factor 53, POLR1E, PAF53, PRAF1 |
| Target/Specificity | This POLR1E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-198 amino acids from the Central region of human POLR1E. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | POLR1E Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | POLR1E |
|---|---|
| Synonyms | PAF53 {ECO:0000303|PubMed:24207024}, PRA |
| Function | Component of RNA polymerase I (Pol I), a DNA-dependent RNA polymerase which synthesizes ribosomal RNA precursors using the four ribonucleoside triphosphates as substrates (PubMed:24207024, PubMed:34671025, PubMed:34887565, PubMed:36271492). Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (PubMed:24207024). |
| Cellular Location | Nucleus, nucleolus |
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Provided below are standard protocols that you may find useful for product applications.
Background
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF (By similarity).
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Percipalle, P., et al. EMBO Rep. 7(5):525-530(2006)
Yamamoto, K., et al. Mol. Cell. Biol. 24(14):6338-6349(2004)
Humphray, S.J., et al. Nature 429(6990):369-374(2004)
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