|Application ||WB, E|
|Calculated MW||22484 Da|
|Antigen Region||133-160 aa|
|Other Names||Cdc42 effector protein 2, Binder of Rho GTPases 1, CDC42EP2, BORG1, CEP2|
|Target/Specificity||This CDC42EP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human CDC42EP2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CDC42EP2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts in a CDC42-dependent manner.|
|Cellular Location||Endomembrane system; Peripheral membrane protein. Cytoplasm, cytoskeleton|
|Tissue Location||Highly expressed in the heart. Weakly expressed in the pancreas and liver|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
firstname.lastname@example.org, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. Coexpression of this protein with dominant negative mutant CDC42 protein in fibroblast was found to induce pseudopodia formation, which suggested a role of this protein in actin filament assembly and cell shape control.
Xue, Y., et al. Int. J. Cancer 118(12):2965-2972(2006)
Joberty, G., et al. Nat. Cell Biol. 3(10):861-866(2001)
Hirsch, D.S., et al. J. Biol. Chem. 276(2):875-883(2001)
Joberty, G., et al. Mol. Cell. Biol. 19(10):6585-6597(1999)
Burbelo, P.D., et al. Proc. Natl. Acad. Sci. U.S.A. 96(16):9083-9088(1999)
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at email@example.com.