|Application ||WB, E|
|Calculated MW||22484 Da|
|Antigen Region||133-160 aa|
|Other Names||Cdc42 effector protein 2, Binder of Rho GTPases 1, CDC42EP2, BORG1, CEP2|
|Target/Specificity||This CDC42EP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-160 amino acids from the C-terminal region of human CDC42EP2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CDC42EP2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts in a CDC42-dependent manner.|
|Cellular Location||Endomembrane system; Peripheral membrane protein. Cytoplasm, cytoskeleton|
|Tissue Location||Highly expressed in the heart. Weakly expressed in the pancreas and liver|
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Provided below are standard protocols that you may find useful for product applications.
CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. Coexpression of this protein with dominant negative mutant CDC42 protein in fibroblast was found to induce pseudopodia formation, which suggested a role of this protein in actin filament assembly and cell shape control.
Xue, Y., et al. Int. J. Cancer 118(12):2965-2972(2006)
Joberty, G., et al. Nat. Cell Biol. 3(10):861-866(2001)
Hirsch, D.S., et al. J. Biol. Chem. 276(2):875-883(2001)
Joberty, G., et al. Mol. Cell. Biol. 19(10):6585-6597(1999)
Burbelo, P.D., et al. Proc. Natl. Acad. Sci. U.S.A. 96(16):9083-9088(1999)
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