|Application ||WB, E|
|Other Accession||A5GFY4, NP_945327.1|
|Calculated MW||66247 Da|
|Antigen Region||548-576 aa|
|Other Names||Negative elongation factor C/D, NELF-C/D, TH1-like protein, NELFCD, NELFD, TH1, TH1L|
|Target/Specificity||This TH1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 548-576 amino acids from the C-terminal region of human TH1L.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||TH1L Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||NELFD, TH1, TH1L|
|Function||Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, the NELFA:NELFCD subcomplex binds to ssDNA and ssRNA in a sequence- and structure- dependent manner.|
|Tissue Location||Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal and cardiac muscle, adrenal, thyroid, kidney and pancreas.|
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Provided below are standard protocols that you may find useful for product applications.
The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini.
Zou, W., et al. Cancer Sci. 101(10):2156-2162(2010)
Yang, Y., et al. J. Cell. Biochem. 109(5):1013-1024(2010)
Sun, J., et al. J. Biol. Chem. 285(9):6443-6452(2010)
Pal, P., et al. Croat. Med. J. 50(4):361-369(2009)
Cheng, C., et al. J. Biol. Chem. 284(13):8786-8796(2009)
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