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HNRNPR Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - HNRNPR Antibody (N-term) AP17239a
    All lanes : Anti-HNRNPR Antibody (N-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: HT-29 whole cell lysate Lane 6: MOLT-4 whole cell lysate Lane 7: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71, 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - HNRNPR Antibody (N-term) AP17239a
    All lanes : Anti-HNRNPR Antibody (N-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: A549 whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: MOLT-4 whole cell lysate Lane 6: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - HNRNPR Antibody (N-term) AP17239a
    HNRNPR Antibody (N-term) (Cat. #AP17239a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the HNRNPR antibody detected the HNRNPR protein (arrow).
  • WB - HNRNPR Antibody (N-term) AP17239a
    Western blot analysis of HNRNPR (arrow) using rabbit polyclonal HNRNPR Antibody (N-term) (Cat. #AP17239a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HNRNPR gene.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O43390
Other Accession NP_001095868.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 70943 Da
Antigen Region 95-123 aa
Additional Information
Gene ID 10236
Other Names Heterogeneous nuclear ribonucleoprotein R, hnRNP R, HNRNPR, HNRPR
Target/Specificity This HNRNPR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 95-123 amino acids from the N-terminal region of human HNRNPR.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHNRNPR Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HNRNPR
Synonyms HNRPR
Function Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus.
Cellular Location Nucleus, nucleoplasm. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Citations (0)

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Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs and also contains a nuclear localization motif. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].

References

Hadian, K., et al. J. Biol. Chem. 284(48):33384-33391(2009)
Fukuda, A., et al. J. Biol. Chem. 284(35):23472-23480(2009)
Tonevitsky, E.A., et al. Bull. Exp. Biol. Med. 147(6):733-736(2009)
Wu, C., et al. Proteomics 7(11):1775-1785(2007)
Szafranski, K., et al. Genome Biol. 8 (8), R154 (2007) :

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Cat# AP17239a
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