SRRT Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
---|---|
Primary Accession | Q9BXP5 |
Other Accession | Q99MR6, A4IFB1, NP_001122325.1, NP_001122324.1 |
Reactivity | Human |
Predicted | Bovine, Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 100666 Da |
Antigen Region | 54-81 aa |
Gene ID | 51593 |
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Other Names | Serrate RNA effector molecule homolog, Arsenite-resistance protein 2, SRRT, ARS2, ASR2 |
Target/Specificity | This SRRT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-81 amino acids from the N-terminal region of human SRRT. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | SRRT Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | SRRT |
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Synonyms | ARS2, ASR2 |
Function | Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and DROSHA, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity. Independently of its activity on miRNAs, necessary and sufficient to promote neural stem cell self- renewal. Does so by directly binding SOX2 promoter and positively regulating its transcription (By similarity). |
Cellular Location | Nucleus, nucleoplasm. Cytoplasm. Note=Predominantly nuclear. Shuttles between the nucleus and the cytoplasm in a CRM1-dependent way (By similarity) |
Tissue Location | Ubiquitously expressed. |
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Background
SRRT acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and RNASEN, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity.
References
Eijgelsheim, M., et al. Hum. Mol. Genet. 19(19):3885-3894(2010)
Kiriyama, M., et al. Mol. Cell. Biol. 29(17):4729-4741(2009)
Gruber, J.J., et al. Cell 138(2):328-339(2009)
Wilson, M.D., et al. Mol. Cell. Biol. 28(5):1503-1514(2008)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
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