|Application ||WB, E|
|Other Accession||NP_002378.1, NP_001078846.1|
|Calculated MW||93027 Da|
|Antigen Region||473-501 aa|
|Other Names||Colorectal mutant cancer protein, Protein MCC, MCC|
|Target/Specificity||This MCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-501 amino acids from the Central region of human MCC.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MCC Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b- catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation (PubMed:18591935, PubMed:19555689, PubMed:22480440). Represses the beta-catenin pathway (canonical Wnt signaling pathway) in a CCAR2-dependent manner by sequestering CCAR2 to the cytoplasm, thereby impairing its ability to inhibit SIRT1 which is involved in the deacetylation and negative regulation of beta-catenin (CTNB1) transcriptional activity (PubMed:24824780).|
|Cellular Location||Cell membrane. Cell projection, lamellipodium. Nucleus. Cytoplasm. Note=Colocalizes with actin at the leading edge of polarized cells|
|Tissue Location||Expressed in a variety of tissues.|
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Provided below are standard protocols that you may find useful for product applications.
This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010)
Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010)
Arnaud, C., et al. FEBS Lett. 583(14):2326-2332(2009)
Fukuyama, R., et al. Oncogene 27(46):6044-6055(2008)
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