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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   UNKL Antibody (N-term)   

UNKL Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - UNKL Antibody (N-term) AP17288a
    UNKL Antibody (N-term) (Cat. #AP17288a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the UNKL antibody detected the UNKL protein (arrow).
  • WB - UNKL Antibody (N-term) AP17288a
    Western blot analysis of UNKL (arrow) using rabbit polyclonal UNKL Antibody (N-term) (Cat. #AP17288a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UNKL gene.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9H9P5
Other Accession Q5FWH2, NP_001180317.1, NP_001032202.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 73828 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 64718
Other Names Putative E3 ubiquitin-protein ligase UNKL, 632-, RING finger protein unkempt-like, Zinc finger CCCH domain-containing protein 5-like, UNKL, C16orf28, ZC3H5L, ZC3HDC5L
Target/Specificity This UNKL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human UNKL.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUNKL Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name UNKL
Synonyms C16orf28, ZC3H5L, ZC3HDC5L
Function May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
Cellular Location Isoform 4: Cytoplasm. Nucleus. Note=Isoform 4 is primarily localized in the cytoplasm but has the ability to shuttle between the nucleus and the cytoplasm
Research Areas
Citations (0)

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Background

UNKL may participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.

References

Lores, P., et al. FEBS J. 277(6):1453-1464(2010)
Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005)
Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)
Martin, J., et al. Nature 432(7020):988-994(2004)
Venter, J.C., et al. Science 291(5507):1304-1351(2001)

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$ 295.00
$ 99.00
Cat# AP17288a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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