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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   AOAH Antibody (Center)   

AOAH Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AOAH Antibody (Center) AP17325c
    Anti-AOAH Antibody (Center) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - AOAH Antibody (Center) AP17325c
    Anti-AOAH Antibody (Center) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 65 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - AOAH Antibody (Center) AP17325c
    AOAH Antibody (Center) (Cat. #AP17325c) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the AOAH antibody detected the AOAH protein (arrow).
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P28039
Other Accession NP_001628.1, NP_001170977.1
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 65105 Da
Antigen Region 168-197 aa
Additional Information
Gene ID 313
Other Names Acyloxyacyl hydrolase, Acyloxyacyl hydrolase small subunit, Acyloxyacyl hydrolase large subunit, AOAH
Target/Specificity This AOAH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human AOAH.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAOAH Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name AOAH
Function Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides.
Cellular Location Secreted.
Research Areas
Citations (0)

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Background

This locus encodes both the light and heavy subunits of acyloxyacyl hydrolase. The encoded enzyme catalyzes the hydrolysis of acyloxylacyl-linked fatty acyl chains from bacterial lipopolysaccharides, effectively detoxifying these molecules. The encoded protein may play a role in modulating host inflammatory response to gram-negative bacteria. Alternatively spliced transcript variants have been described.

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Pelak, K., et al. J. Infect. Dis. 201(8):1141-1149(2010)
Barnes, K.C., et al. J. Allergy Clin. Immunol. 118(1):70-77(2006)
Coulthard, M.G., et al. Infect. Immun. 64(5):1510-1515(1996)
Staab, J.F., et al. J. Biol. Chem. 269(38):23736-23742(1994)

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$ 295.00
$ 99.00
Cat# AP17325c
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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