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>   home   >   Products   >   Primary Antibodies   >   KLHL12 Antibody (C-term)   

KLHL12 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - KLHL12 Antibody (C-term) AP17327b
    KLHL12 Antibody (C-term) (Cat. #AP17327b) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the KLHL12 antibody detected the KLHL12 protein (arrow).
  • WB - KLHL12 Antibody (C-term) AP17327b
    KLHL12 Antibody (C-term) (Cat. #AP17327b) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the KLHL12 antibody detected the KLHL12 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q53G59
Other Accession Q6NRH0, Q8R2H4, Q8BZM0, Q5U374, E1B932, NP_067646.1
Reactivity Human, Mouse
Predicted Bovine, Zebrafish, Rat, Xenopus
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 63277 Da
Antigen Region 376-404 aa
Additional Information
Gene ID 59349
Other Names Kelch-like protein 12, CUL3-interacting protein 1, DKIR homolog, hDKIR, KLHL12, C3IP1
Target/Specificity This KLHL12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-404 amino acids from the C-terminal region of human KLHL12.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLHL12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLHL12
Synonyms C3IP1
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4.
Cellular Location Cytoplasmic vesicle, COPII-coated vesicle
Tissue Location Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland
Citations (0)

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Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.


Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009)
Rondou, P., et al. J. Biol. Chem. 283(17):11083-11096(2008)
Lim, J., et al. Cell 125(4):801-814(2006)
Angers, S., et al. Nat. Cell Biol. 8(4):348-357(2006)
Uchida, K., et al. Immunology 116(1):53-63(2005)

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$ 295.00
$ 99.00
Cat# AP17327b
(40 western blots)
Availability: In Stock
Bulk Size
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