|Application ||WB, E|
|Other Accession||Q6NRH0, Q8R2H4, Q8BZM0, Q5U374, E1B932, NP_067646.1|
|Predicted||Bovine, Zebrafish, Rat, Xenopus|
|Calculated MW||63277 Da|
|Antigen Region||376-404 aa|
|Other Names||Kelch-like protein 12, CUL3-interacting protein 1, DKIR homolog, hDKIR, KLHL12, C3IP1|
|Target/Specificity||This KLHL12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-404 amino acids from the C-terminal region of human KLHL12.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||KLHL12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport (PubMed:22358839, PubMed:27565346). The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed:22358839, PubMed:27565346). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3 (PubMed:16547521). The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4 (PubMed:18303015, PubMed:20100572).|
|Cellular Location||Cytoplasmic vesicle, COPII-coated vesicle|
|Tissue Location||Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.
Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009)
Rondou, P., et al. J. Biol. Chem. 283(17):11083-11096(2008)
Lim, J., et al. Cell 125(4):801-814(2006)
Angers, S., et al. Nat. Cell Biol. 8(4):348-357(2006)
Uchida, K., et al. Immunology 116(1):53-63(2005)
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