|Application ||WB, E|
|Other Accession||Q9R0U0, NP_001177935.1, NP_001177934.1|
|Calculated MW||31301 Da|
|Antigen Region||197-225 aa|
|Other Names||Serine/arginine-rich splicing factor 10, 40 kDa SR-repressor protein, SRrp40, FUS-interacting serine-arginine-rich protein 1, Splicing factor SRp38, Splicing factor, arginine/serine-rich 13A, TLS-associated protein with Ser-Arg repeats, TASR, TLS-associated protein with SR repeats, TLS-associated serine-arginine protein, TLS-associated SR protein, SRSF10, FUSIP1, FUSIP2, SFRS13A, TASR|
|Target/Specificity||This FUSIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 197-225 amino acids from the C-terminal region of human FUSIP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FUSIP1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||FUSIP1, FUSIP2, SFRS13A, TASR|
|Function||Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing (PubMed:11684676, PubMed:12419250, PubMed:14765198). Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70 (PubMed:14765198). Required for splicing repression in M-phase cells and after heat shock (PubMed:14765198). Also acts as a splicing factor that specifically promotes exon skipping during alternative splicing (PubMed:26876937). Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937). May be involved in regulation of alternative splicing in neurons, with isoform 1 acting as a positive and isoform 3 as a negative regulator (PubMed:12419250).|
|Cellular Location||Nucleus speckle Cytoplasm|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
This gene product is a member of the serine-arginine (SR) family of proteins, which is involved in constitutive and regulated RNA splicing. Members of this family are characterized by N-terminal RNP1 and RNP2 motifs, which are required for binding to RNA, and multiple C-terminal SR/RS repeats, which are important in mediating association with other cellular proteins. This protein can influence splice site selection of adenovirus E1A pre-mRNA. It interacts with the oncoprotein TLS, and abrogates the influence of TLS on E1A pre-mRNA splicing. This gene has multiple pseudogenes. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. In addition, transcript variants utilizing alternative polyA sites exist.
Manley, J.L., et al. Genes Dev. 24(11):1073-1074(2010)
Ling, I.F., et al. Hum. Mutat. 31(6):702-709(2010)
Shi, Y., et al. Mol. Cell 28(1):79-90(2007)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Lareau, L.F., et al. Nature 446(7138):926-929(2007)
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