|Application ||WB, E|
|Calculated MW||29862 Da|
|Antigen Region||43-71 aa|
|Other Names||Single-strand selective monofunctional uracil DNA glycosylase, 322-, SMUG1|
|Target/Specificity||This SMUG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human SMUG1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SMUG1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5- formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5- hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.|
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Provided below are standard protocols that you may find useful for product applications.
SMUG1 is a glycosylase that removes uracil from single- and double-stranded DNA in nuclear chromatin, thus contributing to base excision repair.
Arora, M., et al. Leukemia 24(8):1470-1475(2010)
Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)
Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Chanson, A., et al. Am. J. Clin. Nutr. 89(6):1927-1936(2009)
Knaevelsrud, I., et al. Int. J. Radiat. Biol. 85(5):413-420(2009)
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