|Application ||WB, E|
|Other Accession||NP_001091996.1, NP_694938.2|
|Calculated MW||44339 Da|
|Antigen Region||43-72 aa|
|Other Names||Junctional adhesion molecule-like, Adhesion molecule interacting with CXADR antigen 1, Dendritic cell-specific protein CREA7-1, AMICA1, JAML|
|Target/Specificity||This AMICA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-72 amino acids from the N-terminal region of human AMICA1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AMICA1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transmembrane protein of the plasma membrane of leukocytes that control their migration and activation through interaction with CXADR, a plasma membrane receptor found on adjacent epithelial and endothelial cells. The interaction between both receptors mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, JAML induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair. It also controls the transmigration of leukocytes within epithelial and endothelial tissues through adhesive interactions with epithelial and endothelial CXADR.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Cell junction. Note=Localized at the plasma membrane and enriched in areas of cell-cell contacts (PubMed:12869515)|
|Tissue Location||Expression is restricted to the hematopoietic tissues with the exception of liver. Expressed in fetal liver, spleen and thymus. Preferentially expressed by mature leukocytes (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
AMICA1 may function in transmigration of leukocytes through epithelial and endothelial tissues. Expressed at the plasma membrane of polymorphonuclear leukocytes, it mediates adhesive interactions with CXADR, a protein of the junctional complex of epithelial cells. Enhances myeloid leukemia cell adhesion to endothelial cells.
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Luissint, A.C., et al. J. Cell Biol. 183(6):1159-1173(2008)
Zen, K., et al. Mol. Biol. Cell 16(6):2694-2703(2005)
Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004)
Moog-Lutz, C., et al. Blood 102(9):3371-3378(2003)
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