|Application ||WB, E|
|Other Accession||NP_653204.1, NP_751907.1|
|Calculated MW||41253 Da|
|Antigen Region||192-219 aa|
|Other Names||NADPH oxidase organizer 1, NADPH oxidase regulatory protein, Nox organizer 1, Nox-organizing protein 1, SH3 and PX domain-containing protein 5, NOXO1, P41NOX, SH3PXD5|
|Target/Specificity||This NOXO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-219 amino acids from the Central region of human NOXO1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NOXO1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.|
|Cellular Location||Isoform 3: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Isoform 3 associates with the plasma membrane in a lipid-dependent manner (PubMed:12716910)|
|Tissue Location||Expressed in testis, small and large intestines, liver, kidney and pancreas. Isoform 3 is mainly expressed in colon. Isoform 1 is preferentially expressed in testis.|
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Provided below are standard protocols that you may find useful for product applications.
NADPH oxidases (NOXs) catalyze the transfer of electrons from NADPH to molecular oxygen to generate reactive oxygen species (ROS). NOX organizers, such as NOXO1, target NOX activators (see NOXA1; MIM 611255) to NOX and also target NOX to different subcellular compartments (Opitz et al., 2007 [PubMed 17189823]).
Dutta, S., et al. PLoS ONE 5 (5), E10478 (2010) :
Opitz, N., et al. Free Radic. Biol. Med. 42(2):175-179(2007)
Yamamoto, A., et al. Biochem. Biophys. Res. Commun. 352(2):560-565(2007)
Takeya, R., et al. FEBS J. 273(16):3663-3677(2006)
Cheng, G., et al. J. Biol. Chem. 281(26):17718-17726(2006)
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