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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   KLHL2 Antibody (N-term)   

KLHL2 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - KLHL2 Antibody (N-term) AP17585a
    KLHL2 Antibody (N-term) (Cat. #AP17585a) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the KLHL2 antibody detected the KLHL2 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O95198
Other Accession F1LZF0, Q8JZP3, NP_001154993.1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 77-103 aa
Additional Information
Other Names Kelch-like protein 2, Actin-binding protein Mayven, KLHL2
Target/Specificity This KLHL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-103 amino acids from the N-terminal region of human KLHL2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsKLHL2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name KLHL2
Function Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, such as NPTXR, leading most often to their proteasomal degradation (By similarity). Responsible for degradative ubiquitination of the WNK kinases WNK1, WNK3 and WNK4. Plays a role in the reorganization of the actin cytoskeleton. Promotes growth of cell projections in oligodendrocyte precursors.
Cellular Location Cytoplasm, cytoskeleton. Cell projection, ruffle. Cell projection. Cell projection, lamellipodium. Cytoplasm, cytosol. Note=A proportion colocalizes with the actin cytoskeleton. When over-expressed, colocalizes with NPTXR in perinuclear aggresomes (By similarity)
Tissue Location Ubiquitous. Detected throughout the brain. EMBL; AF059569; AAC67502.1; -; mRNA EMBL; AK294103; BAG57438.1; -; mRNA EMBL; AK315372; BAG37765.1; -; mRNA EMBL; AC012504; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC055120; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; AC107059; -; NOT_ANNOTATED_CDS; Genomic_DNA EMBL; BC022503; AAH22503.1; -; mRNA EMBL; BC036468; AAH36468.1; -; mRNA CCDS; CCDS34094.1; -. [O95198-1] CCDS; CCDS54815.1; -. [O95198-2] CCDS; CCDS54816.1; -. [O95198-3] RefSeq; NP_001154993.1; NM_001161521.1. [O95198-2] RefSeq; NP_001154994.1; NM_001161522.1. [O95198-3] RefSeq; NP_001317952.1; NM_001331023.1 RefSeq; NP_001317953.1; NM_001331024.1 RefSeq; NP_009177.3; NM_007246.3. [O95198-1] RefSeq; XP_016863165.1; XM_017007676.1. [O95198-3] UniGene; Hs.388668; - PDB; 2XN4; X-ray; 1.99 A; A/B=294-591 PDB; 4CHB; X-ray; 1.56 A; A/B=294-591 PDBsum; 2XN4; - PDBsum; 4CHB; - ProteinModelPortal; O95198; - SMR; O95198; - BioGrid; 116431; 75 ELM; O95198; - IntAct; O95198; 35 MINT; O95198; - STRING; 9606.ENSP00000424198; - iPTMnet; O95198; - PhosphoSitePlus; O95198; - BioMuta; KLHL2; - EPD; O95198; - PaxDb; O95198; - PeptideAtlas; O95198; - PRIDE; O95198; - ProteomicsDB; 50705; - ProteomicsDB; 50706; -. [O95198-2] DNASU; 11275; - Ensembl; ENST00000226725; ENSP00000226725; ENSG00000109466. [O95198-1] Ensembl; ENST00000514860; ENSP00000424198; ENSG00000109466. [O95198-2] Ensembl; ENST00000538127; ENSP00000437526; ENSG00000109466. [O95198-3] GeneID; 11275; - KEGG; hsa:11275; - UCSC; uc003irb.4; human. [O95198-1] CTD; 11275; - DisGeNET; 11275; - EuPathDB; HostDB:ENSG00000109466.13; - GeneCards; KLHL2; - H-InvDB; HIX0004470; - H-InvDB; HIX0022043; - HGNC; HGNC:6353; KLHL2 HPA; HPA051637; - MIM; 605774; gene neXtProt; NX_O95198; - OpenTargets; ENSG00000109466; - PharmGKB; PA30143; - eggNOG; KOG4441; Eukaryota eggNOG; ENOG410XNX8; LUCA GeneTree; ENSGT00760000118931; - HOGENOM; HOG000230814; - HOVERGEN; HBG014286; - InParanoid; O95198; - KO; K10443; - OMA; TNAWRQV; - OrthoDB; EOG091G04QJ; - PhylomeDB; O95198; - TreeFam; TF329218; - Reactome; R-HSA-8951664; Neddylation Reactome; R-HSA-983168; Antigen processing: Ubiquitination & Proteasome degradation ChiTaRS; KLHL2; human EvolutionaryTrace; O95198; - GenomeRNAi; 11275; - PRO; PR:O95198; - Proteomes; UP000005640; Chromosome 4 Bgee; ENSG00000109466; - CleanEx; HS_KLHL2; - ExpressionAtlas; O95198; baseline and differential Genevisible; O95198; HS GO; GO:0015629; C:actin cytoskeleton; IDA:UniProtKB GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; ISS:UniProtKB GO; GO:0005737; C:cytoplasm; ISS:UniProtKB GO; GO:0005829; C:cytosol; TAS:Reactome GO; GO:0030027; C:lamellipodium; IEA:UniProtKB-SubCell GO; GO:0001726; C:ruffle; IEA:UniProtKB-SubCell GO; GO:0003779; F:actin binding; IDA:UniProtKB GO; GO:0042802; F:identical protein binding; IPI:IntAct GO; GO:0043687; P:post-translational protein modification; TAS:Reactome GO; GO:0016567; P:protein ubiquitination; ISS:UniProtKB Gene3D; 2.120.10.80; -; 1 InterPro; IPR011705; BACK InterPro; IPR017096; BTB-kelch_protein InterPro; IPR000210; BTB/POZ_dom InterPro; IPR015915; Kelch-typ_b-propeller InterPro; IPR006652; Kelch_1 InterPro; IPR011333; SKP1/BTB/POZ_sf Pfam; PF07707; BACK; 1 Pfam; PF00651; BTB; 1 Pfam; PF01344; Kelch_1; 6 PIRSF; PIRSF037037; Kelch-like_protein_gigaxonin; 1 SMART; SM00875; BACK; 1 SMART; SM00225; BTB; 1 SMART; SM00612; Kelch; 6 SUPFAM; SSF117281; SSF117281; 1 SUPFAM; SSF54695; SSF54695; 1 PROSITE; PS50097; BTB; 1 1: Evidence at protein level; 3D-structure; Actin-binding; Alternative splicing; Cell projection; Complete proteome; Cytoplasm; Cytoskeleton; Kelch repeat; Reference proteome; Repeat; Ubl conjugation pathway CHAIN 1 593 Kelch-like protein 2 /FTId=PRO_0000119101 DOMAIN 56 123 BTB. {ECO:0000255|PROSITE- ProRule:PRU00037} REPEAT 308 353 Kelch 1 REPEAT 354 400 Kelch 2 REPEAT 402 447 Kelch 3 REPEAT 449 496 Kelch 4 REPEAT 497 543 Kelch 5 REPEAT 545 591 Kelch 6 VAR_SEQ 1 88 Missing (in isoform 3). /FTId=VSP_047004 VAR_SEQ 1 9 METPPLPPA -> MVWLEARPQILFV (in isoform 2). /FTId=VSP_042837 CONFLICT 273 273 D -> N (in Ref. 1; AAC67502) CONFLICT 342 342 R -> G (in Ref. 4; AAH36468) CONFLICT 457 457 G -> V (in Ref. 4; AAH22503) CONFLICT 462 462 C -> Y (in Ref. 1; AAC67502) CONFLICT 592 592 P -> R (in Ref. 2; BAG37765 and 4; AAH22503). STRAND 307 312 {ECO:0000244|PDB:4CHB} STRAND 315 320 {ECO:0000244|PDB:4CHB} STRAND 323 327 {ECO:0000244|PDB:4CHB} TURN 328 331 {ECO:0000244|PDB:4CHB} STRAND 332 335 {ECO:0000244|PDB:4CHB} STRAND 343 345 {ECO:0000244|PDB:4CHB} STRAND 347 351 {ECO:0000244|PDB:4CHB} STRAND 354 358 {ECO:0000244|PDB:4CHB} STRAND 363 366 {ECO:0000244|PDB:4CHB} STRAND 370 374 {ECO:0000244|PDB:4CHB} TURN 375 378 {ECO:0000244|PDB:4CHB} STRAND 379 383 {ECO:0000244|PDB:4CHB} STRAND 394 398 {ECO:0000244|PDB:4CHB} STRAND 401 405 {ECO:0000244|PDB:4CHB} STRAND 410 413 {ECO:0000244|PDB:4CHB} STRAND 417 421 {ECO:0000244|PDB:4CHB} TURN 422 425 {ECO:0000244|PDB:4CHB} STRAND 426 430 {ECO:0000244|PDB:4CHB} STRAND 441 445 {ECO:0000244|PDB:4CHB} STRAND 448 452 {ECO:0000244|PDB:4CHB} TURN 457 460 {ECO:0000244|PDB:4CHB} STRAND 466 470 {ECO:0000244|PDB:4CHB} TURN 471 474 {ECO:0000244|PDB:4CHB} STRAND 475 478 {ECO:0000244|PDB:4CHB} STRAND 490 494 {ECO:0000244|PDB:4CHB} STRAND 497 501 {ECO:0000244|PDB:4CHB} STRAND 504 509 {ECO:0000244|PDB:2XN4} STRAND 513 516 {ECO:0000244|PDB:4CHB} TURN 518 520 {ECO:0000244|PDB:4CHB} STRAND 523 525 {ECO:0000244|PDB:4CHB} STRAND 537 541 {ECO:0000244|PDB:4CHB} STRAND 544 548 {ECO:0000244|PDB:4CHB} STRAND 553 556 {ECO:0000244|PDB:4CHB} STRAND 560 564 {ECO:0000244|PDB:4CHB} TURN 565 568 {ECO:0000244|PDB:4CHB} STRAND 569 572 {ECO:0000244|PDB:4CHB} STRAND 585 590 {ECO:0000244|PDB:4CHB} SEQUENCE 593 AA; 65975 MW; 397AE02E69E56A18 CRC64; METPPLPPAC TKQGHQKPLD SKDDNTEKHC PVTVNPWHMK KAFKVMNELR SQNLLCDVTI VAEDMEISAH RVVLAACSPY FHAMFTGEMS ESRAKRVRIK EVDGWTLRML IDYVYTAEIQ VTEENVQVLL PAAGLLQLQD VKKTCCEFLE SQLHPVNCLG IRAFADMHAC TDLLNKANTY AEQHFADVVL SEEFLNLGIE QVCSLISSDK LTISSEEKVF EAVIAWVNHD KDVRQEFMAR LMEHVRLPLL PREYLVQRVE EEALVKNSSA CKDYLIEAMK YHLLPTEQRI LMKSVRTRLR TPMNLPKLMV VVGGQAPKAI RSVECYDFKE ERWHQVAELP SRRCRAGMVY MAGLVFAVGG FNGSLRVRTV DSYDPVKDQW TSVANMRDRR STLGAAVLNG LLYAVGGFDG STGLSSVEAY NIKSNEWFHV APMNTRRSSV GVGVVGGLLY AVGGYDGASR QCLSTVECYN ATTNEWTYIA EMSTRRSGAG VGVLNNLLYA VGGHDGPLVR KSVEVYDPTT NAWRQVADMN MCRRNAGVCA VNGLLYVVGG DDGSCNLASV EYYNPTTDKW TVVSSCMSTG RSYAGVTVID KPL
Research Areas
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Background

KLHL2 may play a role in organizing the actin cytoskeleton of the brain cells.

References

Rose, J. Phd, et al. Mol. Med. (2010) In press :
Williams, S.K., et al. J. Neurosci. Res. 81(5):622-631(2005)
Bu, X., et al. Oncogene 24(14):2398-2409(2005)
Soltysik-Espanola, M., et al. Mol. Biol. Cell 10(7):2361-2375(1999)

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$ 295.00
$ 99.00
Cat# AP17585a
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