- CITATIONS: 3
|Application ||WB, E|
|Other Accession||Q8VHQ3, Q95N27, NP_001166206.1|
|Calculated MW||63551 Da|
|Antigen Region||372-399 aa|
|Other Names||Protein phosphatase 1 regulatory inhibitor subunit 16B, Ankyrin repeat domain-containing protein 4, CAAX box protein TIMAP, TGF-beta-inhibited membrane-associated protein, hTIMAP, PPP1R16B, ANKRD4, KIAA0823|
|Target/Specificity||This PPP1R16B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-399 amino acids from the Central region of human PPP1R16B.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PPP1R16B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function. Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation. Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1. Involved in the regulation of endothelial cell filopodia extension. May be a downstream target for TGF-beta1 signaling cascade in endothelial cells.|
|Cellular Location||Cell membrane. Cell membrane; Lipid-anchor. Nucleus. Note=Colocalizes with RPSA/LAMR1 in the cell membrane|
|Tissue Location||Highly expressed in vascular endothelium, CNS, lung, spleen, kidney and testis|
Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
Csortos, C., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (3), L440-L450 (2008) :
Kim, K., et al. Biochem. Biophys. Res. Commun. 338(3):1327-1334(2005)
Homma, K., et al. J. Mol. Biol. 343(5):1207-1220(2004)
Cao, W., et al. Am. J. Physiol., Cell Physiol. 283 (1), C327-C337 (2002) :
Deloukas, P., et al. Nature 414(6866):865-871(2001)
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