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PPP1R16B Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 3
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q96T49 |
| Other Accession | Q8VHQ3, Q95N27, NP_001166206.1 |
| Reactivity | Human |
| Predicted | Bovine, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 63551 Da |
| Antigen Region | 372-399 aa |
| Gene ID | 26051 |
|---|---|
| Other Names | Protein phosphatase 1 regulatory inhibitor subunit 16B, Ankyrin repeat domain-containing protein 4, CAAX box protein TIMAP, TGF-beta-inhibited membrane-associated protein, hTIMAP, PPP1R16B, ANKRD4, KIAA0823 |
| Target/Specificity | This PPP1R16B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-399 amino acids from the Central region of human PPP1R16B. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PPP1R16B Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PPP1R16B |
|---|---|
| Synonyms | ANKRD4, KIAA0823 |
| Function | Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function (PubMed:18586956). Involved in the regulation of the PI3K/AKT signaling pathway, angiogenesis and endothelial cell proliferation (PubMed:25007873). Regulates angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (PubMed:16263087, PubMed:18586956). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation (PubMed:18586956). Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (PubMed:16263087). Involved in the dephosphorylation of EEF1A1 (PubMed:26497934). |
| Cellular Location | Cell membrane. Cell membrane; Lipid-anchor. Nucleus. Cell projection. Note=Colocalizes with RPSA/LAMR1 in the cell membrane (PubMed:16263087). Localizes to the perinuclear region (By similarity). Colocalizes with PTEN at the tip of EC projections (PubMed:25007873). {ECO:0000250|UniProtKB:Q95N27, ECO:0000269|PubMed:16263087, ECO:0000269|PubMed:25007873} |
Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.
References
Csortos, C., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (3), L440-L450 (2008) :
Kim, K., et al. Biochem. Biophys. Res. Commun. 338(3):1327-1334(2005)
Homma, K., et al. J. Mol. Biol. 343(5):1207-1220(2004)
Cao, W., et al. Am. J. Physiol., Cell Physiol. 283 (1), C327-C337 (2002) :
Deloukas, P., et al. Nature 414(6866):865-871(2001)
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