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PDZD3 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q86UT5 |
| Other Accession | NP_001161940.1, NP_079067.3 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 61032 Da |
| Antigen Region | 258-285 aa |
| Gene ID | 79849 |
|---|---|
| Other Names | Na(+)/H(+) exchange regulatory cofactor NHE-RF4, NHERF-4, Intestinal and kidney-enriched PDZ protein, Natrium-phosphate cotransporter IIa C-terminal-associated protein 2, Na/Pi cotransporter C-terminal-associated protein 2, NaPi-Cap2, PDZ domain-containing protein 2, PDZ domain-containing protein 3, Sodium-hydrogen exchanger regulatory factor 4, PDZD3, IKEPP, NHERF4, PDZK2 |
| Target/Specificity | This PDZD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-285 amino acids from the Central region of human PDZD3. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | PDZD3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NHERF4 |
|---|---|
| Synonyms | IKEPP, PDZD3, PDZK2 |
| Function | Acts as a regulatory protein that associates with GUCY2C and negatively modulates its heat-stable enterotoxin-mediated activation (PubMed:11950846). Stimulates SLC9A3 activity in the presence of elevated calcium ions (PubMed:19088451). |
| Cellular Location | Cell membrane; Peripheral membrane protein. Cytoplasm Note=Preferentially accumulates at the apical surface and ileal brush border of intestinal epithelial cells (PubMed:11950846, PubMed:19088451). |
| Tissue Location | Expressed in kidney and the gastrointestinal tract. Not detected in brain, heart, skeletal muscle or cells of hematopoietic origin. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Guanylyl cyclase C (GCC, or GUCY2C; MIM 601330) produces cGMP following the binding of either endogenous ligands or heat-stable enterotoxins secreted by E. coli and other enteric bacteria. Activation of GCC initiates a signaling cascade that leads to phosphorylation of the cystic fibrosis transmembrane conductance regulator (CFTR; MIM 602421), followed by a net efflux of ions and water into the intestinal lumen. IKEPP is a regulatory protein that associates with GCC and regulates the amount of cGMP produced following receptor stimulation (Scott et al., 2002 [PubMed 11950846]).
References
Zachos, N.C., et al. Cell. Physiol. Biochem. 22 (5-6), 693-704 (2008) :
Kato, Y., et al. Mol. Pharmacol. 67(3):734-743(2005)
Hegedus, T., et al. Biochem. Biophys. Res. Commun. 302(3):454-461(2003)
Scott, R.O., et al. J. Biol. Chem. 277(25):22934-22941(2002)
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