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ARGFX Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - ARGFX Antibody (Center) AP17681C
    Fluorescent confocal image of HepG2 cell stained with ARGFX Antibody (Center)(Cat#AP17681c).HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGFX primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ARGFX immunoreactivity is localized to Nucleus significantly.
    detail
  • WB - ARGFX Antibody (Center) AP17681C
    ARGFX Antibody (Center) (Cat. #AP17681c) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the ARGFX antibody detected the ARGFX protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession A6NJG6
Other Accession NP_001012677.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35617 Da
Antigen Region 103-132 aa
Additional Information
Gene ID 503582
Other Names Arginine-fifty homeobox, ARGFX
Target/Specificity This ARGFX antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-132 amino acids from the Central region of human ARGFX.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsARGFX Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ARGFX (HGNC:30146)
Function Transcription factor that acts as activator.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
Tissue Location Expressed at low level in testis and undifferentiated embryonic stem cells.
Citations (0)
citation

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Background

Homeobox genes encode DNA-binding proteins, many of which are thought to be involved in early embryonic development. Homeobox genes encode a DNA-binding domain of 60 to 63 amino acids referred to as the homeodomain. This gene is a member of the ARGFX homeobox gene family.

References

Booth, H.A., et al. Gene 387 (1-2), 7-14 (2007) :

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$ 365.00
$ 140.00
Cat# AP17681C
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