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>   home   >   Products   >   Primary Antibodies   >   TFAP2D Antibody (C-term)   

TFAP2D Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - TFAP2D Antibody (C-term) AP17936B
    Fluorescent image of A549 cell stained with TFAP2D Antibody (C-term)(Cat#AP17936b).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAP2D primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).TFAP2D immunoreactivity is localized to Nucleus significantly.
  • WB - TFAP2D Antibody (C-term) AP17936B
    TFAP2D Antibody (C-term) (Cat. #AP17936b) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the TFAP2D antibody detected the TFAP2D protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB, E
Primary Accession Q7Z6R9
Other Accession Q91ZK0, NP_758438.2
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 49578 Da
Antigen Region 308-336 aa
Additional Information
Gene ID 83741
Other Names Transcription factor AP-2-delta, AP2-delta, Activating enhancer-binding protein 2-delta, Transcription factor AP-2-beta-like 1, TFAP2D (HGNC:15581)
Target/Specificity This TFAP2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the C-terminal region of human TFAP2D.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTFAP2D Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TFAP2D (HGNC:15581)
Function Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC (By similarity).
Cellular Location Nucleus.
Tissue Location Highly expressed in brain, placenta, skeletal muscle, thymus, small intestine, and prostate, and expressed at lower levels in leukocyte, spleen, testis, ovary and colon. Barely detectable in heart, kidney, liver, lung or pancreas
Citations (0)

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Background

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC (By similarity).

References

Kim, J.M., et al. DNA Res. 13(6):275-286(2006)
Wenke, A.K., et al. Biochem. Biophys. Res. Commun. 345(1):495-501(2006)
Cheng, C., et al. Int. J. Biochem. Cell Biol. 34(1):78-86(2002)

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Cat# AP17936B
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(40 western blots)
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