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AGPAT9 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - AGPAT9 Antibody (Center) AP18013c
    AGPAT9 Antibody (Center) (Cat. #AP18013c) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the AGPAT9 antibody detected the AGPAT9 protein (arrow).
    detail
  • WB - AGPAT9 Antibody (Center) AP18013c
    AGPAT9 Antibody (Center) (Cat. #AP18013c) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the AGPAT9 antibody detected the AGPAT9 protein (arrow).
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q53EU6
Other Accession Q4V8J4, Q8C0N2, NP_116106.2
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 48705 Da
Antigen Region 194-222 aa
Additional Information
Gene ID 84803
Other Names Glycerol-3-phosphate acyltransferase 3, GPAT-3, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10, AGPAT 10, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9, 1-AGP acyltransferase 9, 1-AGPAT 9, Acyl-CoA:glycerol-3-phosphate acyltransferase 3, hGPAT3, Lung cancer metastasis-associated protein 1, Lysophosphatidic acid acyltransferase theta, LPAAT-theta, MAG-1, AGPAT9, GPAT3, MAG1
Target/Specificity This AGPAT9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-222 amino acids from the Central region of human AGPAT9.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsAGPAT9 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPAT3 (HGNC:28157)
Synonyms AGPAT9, MAG1
Function Converts glycerol-3-phosphate to 1-acyl-sn-glycerol-3- phosphate (lysophosphatidic acid or LPA) by incorporating an acyl moiety at the sn-1 position of the glycerol backbone (PubMed:17170135). Also converts LPA into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (PubMed:19318427). Protects cells against lipotoxicity (PubMed:30846318).
Cellular Location Endoplasmic reticulum membrane; Multi-pass membrane protein
Tissue Location Widely expressed. Expressed in liver, kidney, testis, brain, heart, skeletal muscle, thyroid, prostate, thymus and placenta. Also expressed lung and adipose tissue
Research Areas
Citations (0)
citation

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Background

Glycerol-3-phosphate (G3P) acyltransferases (GPAT; EC 2.3.1.15), such as GPAM (MIM 602395) and GPAT3, catalyze the initial step of de novo triacylglycerol (TAG) synthesis by converting glycerol-3-phosphate (G3P) to lysophosphatidic acid (LPA) (Cao et al., 2006 [PubMed 17170135]).

References

Shan, D., et al. J. Lipid Res. 51(7):1971-1981(2010)
Cao, J., et al. Proc. Natl. Acad. Sci. U.S.A. 103(52):19695-19700(2006)
Tang, W., et al. J. Biochem. Mol. Biol. 39(5):626-635(2006)
Yamada, S., et al. Oncogene 23(35):5901-5911(2004)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

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$ 365.00
$ 140.00
Cat# AP18013c
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