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ATG4B AntibodyPeptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1809d | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ATG4B Antibody - Product info | |
| Application | WB, IHC
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| Primary Accession | Q9Y4P1 |
| Other Accession | Q8BGE6, Q6DG88, Q6PZ02, Q6PZ03 |
| Reactivity | Human |
| Predicted | Bovine, Chicken, Mouse, Zebrafish |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 44294 Da |
ATG4B Antibody - Additional info | |
| Gene ID 23192 | |
| Other Names ATG4B; APG4B; AUTL1; KIAA0943; Cysteine protease ATG4B; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B | |
| Target/Specificity This ATG4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264~293 amino acids from the central region of human APG4B. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ATG4B Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
ATG4B Antibody - Protein Information | |
| Name ATG4B | |
| Synonyms APG4B, AUTL1, KIAA0943 | |
| Function Cysteine protease required for autophagy, which cleaves the C-terminal part of MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Also mediates the lipid deconjugation required for target recycling | |
| Cellular Location Cytoplasm (Probable). | |
| Tissue Location Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas | |
ATG4B Antibody - Related products
AP1809a: ATG4B Antibody (N-term)
AP1809c: ATG4B Antibody (C-term)
AP3560a: Phospho-ATG4B-S121 Antibody
AP3561a: Phospho-ATG4B-S309 Antibody
RI10455: ATG4B predesign siRNA
BP1809a: APG4B Antibody (N-term) Blocking Peptide
BP1809b: APG4B Antibody (G93) Blocking Peptide
BP1809c: APG4B Antibody (C-term) Blocking Peptide
BP1809d: APG4B Antibody (E273) Blocking Peptide
BP1809e: APG4B Antibody (E135) Blocking Peptide
BP1809f: APG4B Antibody (S309) Blocking Peptide
BP1809g: APG4B Antibody (T69) Blocking Peptide
BP1809h: APG4B Antibody (G254) Blocking Peptide
BP1809i: APG4B Antibody (S121) Blocking Peptide
ATG4B Antibody - Application data
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Western blot analysis of APG4B (arrow) using purified Pab (Cat.#AP1809d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the APG4B gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human skeletal muscle reacted with Autophagy APG4B Antibody (E273)(Cat.#AP1809d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
ATG4B Antibody - Research Areas
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BACKGROUND
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4, a cysteine protease required for autophagy, cleaves the C-terminal part of MAP1LC3 to form the activated molecule LC3-I. LC3-I is subsequently conjugated with phosphatidylethanolamine at the C-terminal glycine to form LC3-II, a marker for autophagy via its capacity to bind to autophagosomes.
REFERENCES
Tanida, I., et al., J. Biol. Chem. 279(35):36268-36276 (2004). Marino, G., et al., J. Biol. Chem. 278(6):3671-3678 (2003). Kabeya, Y., et al., J. Cell. Sci. 117 (Pt 13), 2805-2812 (2004) (): ().
