PPIL5 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q96L50 |
Other Accession | NP_689542.2 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 46723 Da |
Antigen Region | 127-153 aa |
Gene ID | 122769 |
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Other Names | Leucine-rich repeat protein 1, 4-1BB-mediated-signaling molecule, 4-1BBlrr, LRR-repeat protein 1, LRR-1, Peptidylprolyl isomerase-like 5, LRR1, PPIL5 |
Target/Specificity | This PPIL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 127-153 amino acids from the Central region of human PPIL5. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | PPIL5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | LRR1 (HGNC:19742) |
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Synonyms | PPIL5 |
Function | Substrate recognition subunit of an ECS (Elongin BC-CUL2/5- SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:15601820). ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S- phase (By similarity). May negatively regulate the 4-1BB-mediated signaling cascades which result in the activation of NK-kappaB and JNK1 (PubMed:11804328). |
Cellular Location | Nucleus. |
Tissue Location | Ubiquitous. Maximal expression was seen in the heart and skeletal muscle and minimal expression seen in the kidney |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene contains a leucine-rich repeat (LRR). It specifically interacts with TNFRSF9/4-1BB, a member of the tumor necrosis factor receptor (TNFR) superfamily. Overexpression of this gene suppresses the activation of NF-kappa B induced by TNFRSF9 or TNF receptor-associated factor 2 (TRAF2), which suggests that this protein is a negative regulator of TNFRSF9-mediated signaling cascades. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.
References
Jang, L.K., et al. Mol. Cells 12(3):304-312(2001)
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