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ATG4C Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1810b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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ATG4C Antibody (Center) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q96DT6 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 52497 Da |
ATG4C Antibody (Center) - Additional info | |
| Gene ID 84938 | |
| Other Names ATG4C; APG4C; AUTL1; AUTL3; Cysteine protease ATG4C; AUT-like 3 cysteine endopeptidase; Autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C | |
| Target/Specificity This ATG4C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174~204 amino acids from the Center of human APG4C. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ATG4C Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ATG4C Antibody (Center) - Protein Information | |
| Name ATG4C | |
| Synonyms APG4C, AUTL1, AUTL3 | |
| Function Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes | |
| Cellular Location Cytoplasm (Probable). | |
| Tissue Location Highly expressed in skeletal muscle, heart, liver and testis | |
ATG4C Antibody (Center) - Related products
AP1810a: ATG4C Antibody (N-term)
AP1810b: ATG4C Antibody (Center)
AP1810c: ATG4C Antibody (C-term)
AP3364a: Phospho-ATG4C-S451 Antibody
AP3392a: Phospho-ATG4C-S177 Antibody
AP3440a: Phospho-ATG4C-S398 Antibody
AP3456a: Phospho-ATG4C-S166 Antibody
RI10456: ATG4C predesign siRNA
LY10583a: ATG4C Over-expression Lysate
BP1810a: APG4C Antibody (N-term) Blocking Peptide
BP1810b: APG4C Antibody (Center) Blocking Peptide
BP1810c: APG4C Antibody (C-term) Blocking Peptide
BP1810d: APG4C Antibody (S398) Blocking Peptide
BP1810e: APG4C Antibody (S166) Blocking Peptide
BP3364a: Phospho-APG4C-S451 Antibody Blocking Peptide
BP3392a: Phospho-APG4C-S177 Antibody Blocking Peptide
ATG4C Antibody (Center) - Application data
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Western blot analysis of anti-hAPG4C-I182 Pab (Cat. #AP1810b) in 293 cell line lysates transiently transfected with the ATG4C gene (2ug/lane). hAPG4C-I182(arrow) was detected using the purified Pab (1:60 dilution).
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The anti-APG4C Pab (Cat. #AP1810b) is used in Western blot to detect APG4C in Hela tissue lysate
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
ATG4C Antibody (Center) - Research Areas
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BACKGROUND
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.
REFERENCES
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
