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DSPP Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - DSPP Antibody (N-term) AP18118a
    All lanes : Anti-DSPP Antibody (N-term) at 1:2000 dilution Lane 1: LNCap whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 131 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - DSPP Antibody (N-term) AP18118a
    DSPP Antibody (N-term) (Cat. #AP18118a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DSPP antibody detected the DSPP protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NZW4
Other Accession NP_055023.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 131151 Da
Antigen Region 47-76 aa
Additional Information
Gene ID 1834
Other Names Dentin sialophosphoprotein, Dentin phosphoprotein, Dentin phosphophoryn, DPP, Dentin sialoprotein, DSP, DSPP
Target/Specificity This DSPP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 47-76 amino acids of human DSPP.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDSPP Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DSPP
Function DSP may be an important factor in dentinogenesis. DPP may bind high amount of calcium and facilitate initial mineralization of dentin matrix collagen as well as regulate the size and shape of the crystals.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Expressed in teeth. DPP is synthesized by odontoblast and transiently expressed by pre-ameloblasts
Research Areas
Citations (0)

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Background

This gene encodes two principal proteins of the dentin extracellular matrix of the tooth. The preproprotein is secreted by odontoblasts and cleaved into dentin sialoprotein and dentin phosphoprotein. Dentin phosphoprotein is thought to be involved in the biomineralization process of dentin. Mutations in this gene have been associated with dentinogenesis imperfecta-1; in some individuals, dentinogenesis imperfecta occurs in combination with an autosomal dominant form of deafness. Allelic differences due to repeat polymorphisms have been found for this gene. [provided by RefSeq].

References

Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Bai, H., et al. BMC Med. Genet. 11, 23 (2010) :
Kida, M., et al. Eur. J. Oral Sci. 117(6):691-694(2009)
Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009) :
Qu, E.J., et al. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 26(5):536-538(2009)

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$ 295.00
$ 99.00
Cat# AP18118a
Size:
Quantity:
(40 western blots)
Availability: In Stock
Bulk Size
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