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APG5 AntibodyPurified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP1812c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP1812c-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 1
- Reviews
- Protocols
- Backgrounds
APG5 Antibody - Product info | |
| Application | IF, WB, IHC
|
| Primary Accession | Q9H1Y0 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 32447 Da |
APG5 Antibody - Additional info | |
| Gene ID 9474 | |
| Other Names ATG5; APG5L; ASP; Autophagy protein 5; APG5-like; Apoptosis-specific protein | |
| Target/Specificity This APG5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the full length of human APG5. | |
| Dilution IF~~1:200 WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions APG5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. | |
APG5 Antibody - Protein Information | |
| Name ATG5 | |
| Synonyms APG5L, ASP | |
| Function Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity) | |
| Cellular Location Cytoplasm. Note=Colocalizes with nonmuscle actin | |
| Tissue Location Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells | |
APG5 Antibody - Related products
AP1812a: ATG5 Antibody (N-term)
AP1812b: ATG5 Antibody (C-term)
DC01069: Human ATG5 cDNA Clone
LY11706a: ATG5 Over-expression Lysate
APG5 Antibody - Application data
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Fluorescent image of U251 cells stained with APG5 antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP1812c APG5 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). ATG5 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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APG5 Antibody (Cat.#AP1812c) western blot analysis in UM cell line lysates (35ug/lane).This demonstrates the APG5 antibody detected the APG5 protein (arrow).
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APG5 Antibody (Cat. #AP1812cb)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG5 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
APG5 Antibody - Research Areas
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BACKGROUND
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG5, required for autophagy, conjugates to ATG12 and associates with an isolation membrane to form a cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. APG5 may also play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
REFERENCES
References for protein: 1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) 2. Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) 3.Greenberg JT. Dev Cell. 8(6):799-801. (2005) 4.Levine B. Cell. 120(2):159-62. (2005) 5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) 6. Hammond E.M., et al. FEBS Lett. 425:391-395(1998) 7.Strausberg R.L., et al. PNAS 99:16899-16903(2002) 8.Grand R.J.A., et al. Exp. Cell Res. 218:439-451(1995) 9. Mizushima N., et al. J. Biol. Chem. 273:33889-33892(1998) 10. Mizushima N., et al. J. Cell Biol. 152:657-668(2001) References for U251 cell line: 1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].
