|Application ||WB, E|
|Other Accession||Q91WF7, NP_055660.1|
|Calculated MW||103635 Da|
|Antigen Region||846-874 aa|
|Other Names||Polyphosphoinositide phosphatase, 313-, Phosphatidylinositol 3, 5-bisphosphate 5-phosphatase, SAC domain-containing protein 3, FIG4, KIAA0274, SAC3|
|Target/Specificity||This FIG4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 846-874 amino acids from the C-terminal region of human FIG4.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||FIG4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D5-phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 > PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.|
|Cellular Location||Endosome membrane. Note=Localization requires VAC14 and PIKFYVE|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease, type 4J.
Ikonomov, O.C., et al. J. Biol. Chem. 285(35):26760-26764(2010)
Tsai, C.P., et al. Neurobiol. Aging (2010) In press :
Ikonomov, O.C., et al. J. Biol. Chem. 284(51):35794-35806(2009)
Soranzo, N., et al. Nat. Genet. 41(11):1182-1190(2009)
Trynka, G., et al. Gut 58(8):1078-1083(2009)
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