|Application ||WB, E|
|Calculated MW||128153 Da|
|Antigen Region||641-669 aa|
|Other Names||Nck-associated protein 1-like, Hematopoietic protein 1, Membrane-associated protein HEM-1, NCKAP1L, HEM1|
|Target/Specificity||This NCKPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 641-669 amino acids from the Central region of human NCKPL.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NCKPL Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages (PubMed:16417406, PubMed:17696648). Component of the WAVE2 complex which signals downstream of RAC to stimulate F- actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (By similarity). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin (PubMed:16417406). Also involved in mechanisms WAVE- independent to regulate myosin and actin polymerization during neutrophil chemotaxis (PubMed:17696648).|
|Cellular Location||Cell membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm Note=Localizes to the leading edge of polarized neutrophils|
|Tissue Location||Expressed only in cells of hematopoietic origin (PubMed:7643388, PubMed:1932118). Expressed in neutrophils (at protein level) (PubMed:16417406)|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.
Joshi, A.D., et al. Clin. Cancer Res. 13 (18 PT 1), 5295-5304 (2007) :
Weiner, O.D., et al. PLoS Biol. 5 (9), E221 (2007) :
Weiner, O.D., et al. PLoS Biol. 4 (2), E38 (2006) :
Baumgartner, S., et al. J. Mol. Biol. 251(1):41-49(1995)
Hromas, R., et al. Biochim. Biophys. Acta 1090(2):241-244(1991)
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