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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NCKPL Antibody (Center)   

NCKPL Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NCKPL Antibody (Center) AP18156c
    NCKPL Antibody (Center) (Cat. #AP18156c) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the NCKPL antibody detected the NCKPL protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P55160
Other Accession NP_005328.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 128153 Da
Antigen Region 641-669 aa
Additional Information
Gene ID 3071
Other Names Nck-associated protein 1-like, Hematopoietic protein 1, Membrane-associated protein HEM-1, NCKAP1L, HEM1
Target/Specificity This NCKPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 641-669 amino acids from the Central region of human NCKPL.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNCKPL Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NCKAP1L (HGNC:4862)
Function Essential hematopoietic-specific regulator of the actin cytoskeleton (Probable). Controls lymphocyte development, activation, proliferation and homeostasis, erythrocyte membrane stability, as well as phagocytosis and migration by neutrophils and macrophages (PubMed:16417406, PubMed:17696648). Component of the WAVE2 complex which signals downstream of RAC to stimulate F- actin polymerization. Required for stabilization and/or translation of the WAVE2 complex proteins in hematopoietic cells (By similarity). Exhibits complex cycles of activation and inhibition to generate waves of propagating the assembly with actin (PubMed:16417406). Also involved in mechanisms WAVE- independent to regulate myosin and actin polymerization during neutrophil chemotaxis (PubMed:17696648).
Cellular Location Cell membrane; Single-pass membrane protein; Cytoplasmic side. Cytoplasm Note=Localizes to the leading edge of polarized neutrophils
Tissue Location Expressed only in cells of hematopoietic origin (PubMed:7643388, PubMed:1932118). Expressed in neutrophils (at protein level) (PubMed:16417406)
Research Areas
Citations (0)

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This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.


Joshi, A.D., et al. Clin. Cancer Res. 13 (18 PT 1), 5295-5304 (2007) :
Weiner, O.D., et al. PLoS Biol. 5 (9), E221 (2007) :
Weiner, O.D., et al. PLoS Biol. 4 (2), E38 (2006) :
Baumgartner, S., et al. J. Mol. Biol. 251(1):41-49(1995)
Hromas, R., et al. Biochim. Biophys. Acta 1090(2):241-244(1991)

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$ 295.00
$ 99.00
Cat# AP18156c
(40 western blots)
Availability: In Stock
Bulk Size
Seasonal Special on Bulk Order
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