|Application ||WB, E|
|Other Accession||Q5U316, Q6PHN9, NP_001161078.1|
|Calculated MW||23025 Da|
|Antigen Region||171-199 aa|
|Other Names||Ras-related protein Rab-35, GTP-binding protein RAY, Ras-related protein Rab-1C, RAB35, RAB1C, RAY|
|Target/Specificity||This RAB35 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the C-terminal region of human RAB35.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAB35 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in the process of endocytosis and is an essential rate-limiting regulator of the fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. May indirectly regulate neurite outgrowth. Together with TBC1D13 may be involved in regulation of insulin-induced glucose transporter SLC2A4/GLUT4 translocation to the plasma membrane in adipocytes.|
|Cellular Location||Cell membrane; Lipid-anchor; Cytoplasmic side. Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin- coated vesicle. Endosome Melanosome. Note=Present on sorting endosomes and recycling endosome tubules (PubMed:16950109). Tends to be enriched in PIP2-positive cell membrane domains (PubMed:16950109). During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis (PubMed:16950109). Identified in stage I to stage IV melanosomes (PubMed:17081065).|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
RAB35 possesses GTPase activity.
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Zhang, J., et al. Science 325(5945):1250-1254(2009)
Patino-Lopez, G., et al. J. Biol. Chem. 283(26):18323-18330(2008)
Chi, A., et al. J. Proteome Res. 5(11):3135-3144(2006)
Kouranti, I., et al. Curr. Biol. 16(17):1719-1725(2006)
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