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GPR83 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - GPR83 Antibody (N-term) AP18225a
    GPR83 Antibody (N-term) (Cat. #AP18225a) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the GPR83 antibody detected the GPR83 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession Q9NYM4
Other Accession NP_057624.3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 48339 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 10888
Other Names Probable G-protein coupled receptor 83, G-protein coupled receptor 72, GPR83, GPR72, KIAA1540
Target/Specificity This GPR83 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human GPR83.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGPR83 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GPR83 (HGNC:4523)
Function G-protein coupled receptor for PEN, a neuropeptide produced from the precursor protein, proSAAS (encoded by PCSK1N). Acts through a G(i)- and G(q)-alpha-alpha-mediated pathway in response to PEN (PubMed:27117253). Plays a role in food intake and body weight regulation. May contribute to the regulation of anxiety-related behaviors (By similarity).
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P30731}; Multi-pass membrane protein. Note=Colocalizes with GPR171 in the paraventricular nucleus. Colocalizes with the ghrelin receptor GHSR1A in the hypothalamus. {ECO:0000250|UniProtKB:P30731}
Tissue Location Highly expressed in the brain and spinal cord, and found in lower concentrations in the thymus and other tissues
Research Areas
Citations (0)
citation

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Background

GPR83 is an orphan receptor. Could be a neuropeptide Y receptor.

References

Hansen, W., et al. Genes Immun. 11(4):357-361(2010)
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Hansen, W., et al. J. Immunol. 177(1):209-215(2006)
Parker, R., et al. Biochim. Biophys. Acta 1491 (1-3), 369-375 (2000) :
De Moerlooze, L., et al. Cytogenet. Cell Genet. 90 (1-2), 146-150 (2000) :

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$ 365.00
$ 140.00
Cat# AP18225a
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