- CITATIONS: 4
|Application ||WB, IHC-P, E|
|Calculated MW||44882 Da|
|Antigen Region||125-154 aa|
|Other Names||Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a, LAMP1|
|Target/Specificity||This LAMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-154 amino acids from the N-terminal region of human LAMP1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LAMP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein Lysosome membrane; Single-pass type I membrane protein. Late endosome. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. Colocalizes with OSBPL1A at the late endosome|
Provided below are standard protocols that you may find useful for product applications.
LAMP1 is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.
References for protein:
1.Fukuda M., J. Biol. Chem. 263:18920-18928(1988).
2.Sawada R., J. Biol. Chem. 268:9014-9022(1993).
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].
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