|Application ||WB, E|
|Calculated MW||52083 Da|
|Antigen Region||121-148 aa|
|Other Names||Diphthamide biosynthesis protein 2, DPH2 homolog, HsDph2, Diphthamide biosynthesis protein 2 homolog-like 2, DPH2-like 2, DPH2, DPH2L2|
|Target/Specificity||This DPH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-148 amino acids from the N-terminal region of human DPH2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DPH2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).|
|Tissue Location||Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes|
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Provided below are standard protocols that you may find useful for product applications.
This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Two transcript variants encoding different isoforms have been found for this gene.
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Liu, S., et al. Mol. Cell. Biol. 24(21):9487-9497(2004)
Schultz, D.C., et al. Genomics 52(2):186-191(1998)
Foley, B.T., et al. J. Biol. Chem. 270(39):23218-23225(1995)
Mattheakis, L.C., et al. Gene 132(1):149-154(1993)
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