- CITATIONS: 2
|Application ||WB, IF, E|
|Calculated MW||44346 Da|
|Antigen Region||8-38 aa|
|Other Names||Lysosome-associated membrane glycoprotein 3, LAMP-3, Lysosomal-associated membrane protein 3, DC-lysosome-associated membrane glycoprotein, DC LAMP, Protein TSC403, CD208, LAMP3, DCLAMP, TSC403|
|Target/Specificity||This LAMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-38 amino acids from the N-terminal region of human LAMP3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||LAMP3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in dendritic cell function and in adaptive immunity.|
|Cellular Location||Lysosome membrane; Single-pass type I membrane protein. Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Note=During dendritic cell maturation, detected on cytoplasmic vesicles (the MHC II compartment) that contain MHC II proteins, LAMP1, LAMP2 and LAMP3. Detected on lysosomes in mature dendritic cells|
|Tissue Location||Detected in tonsil interdigitating dendritic cells, in spleen, lymph node, Peyer's patches in the small instestine, in thymus medulla and in B-cells (at protein level) Expressed in lymphoid organs and dendritic cells. Expressed in lung. Up-regulated in carcinomas of the esophagus, colon, rectum, ureter, stomach, breast, fallopian tube, thyroid and parotid tissues.|
Provided below are standard protocols that you may find useful for product applications.
LAMP3 is a member of a family of membrane glycoproteins. It may change lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells.
References for protein:
1.Elliott,B., Clin. Cancer Res. 13 (13), 3825-3830 (2007)
2.Zhu,L.C., Hum. Pathol. 38 (2), 260-268 (2007)
3.Arruda,L.B., J. Immunol. 177 (4), 2265-2275 (2006)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].
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