|Application ||WB, E|
|Calculated MW||52355 Da|
|Antigen Region||195-222 aa|
|Other Names||CUGBP Elav-like family member 5, CELF-5, Bruno-like protein 5, CUG-BP- and ETR-3-like factor 5, RNA-binding protein BRUNOL-5, CELF5, BRUNOL5|
|Target/Specificity||This BRUNOL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 195-222 amino acids from the Central region of human BRUNOL5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||BRUNOL5 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||RNA-binding protein implicated in the regulation of pre- mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA.|
|Cellular Location||Nucleus. Cytoplasm|
|Tissue Location||Expressed in brain.|
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Provided below are standard protocols that you may find useful for product applications.
Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternatively spliced transcript variants have been identified in this gene.
Ladd, A.N., et al. Mol. Cell. Biol. 21(4):1285-1296(2001)
Good, P.J., et al. J. Biol. Chem. 275(37):28583-28592(2000)
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