MED9 Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, E |
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Primary Accession | Q9NWA0 |
Other Accession | Q8VCS6, Q2KHX9, NP_060489.1 |
Reactivity | Human, Mouse |
Predicted | Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 16403 Da |
Antigen Region | 107-136 aa |
Gene ID | 55090 |
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Other Names | Mediator of RNA polymerase II transcription subunit 9, Mediator complex subunit 9, MED9, MED25 |
Target/Specificity | This MED9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-136 amino acids from the C-terminal region of human MED9. |
Dilution | WB~~1:1000 |
Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | MED9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | MED9 |
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Synonyms | MED25 |
Function | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene- specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
Cellular Location | Nucleus. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The multiprotein Mediator complex is a coactivator required for activation of RNA polymerase II transcription by DNA bound transcription factors. The protein encoded by this gene is thought to be a subunit of the Mediator complex. This gene is located within the Smith-Magenis syndrome region on chromosome 17.
References
Sato, S., et al. Mol. Cell 14(5):685-691(2004)
Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
Bi, W., et al. Genome Res. 12(5):713-728(2002)
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