|Application ||WB, E|
|Other Accession||Q3KQD1, Q80ZG5, Q8BHJ9, Q4R4P2, Q5U3F2, Q5ZIG2, Q3ZBE5, NP_006416.3|
|Predicted||Bovine, Chicken, Zebrafish, Monkey, Mouse, Rat, Xenopus|
|Calculated MW||68387 Da|
|Antigen Region||60-86 aa|
|Other Names||Pre-mRNA-splicing factor SLU7, hSlu7, SLU7|
|Target/Specificity||This SLU7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-86 amino acids from the N-terminal region of human SLU7.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SLU7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'- splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.|
|Cellular Location||Nucleus. Cytoplasm. Note=Predominantly nuclear. Shuttling between the nucleus and the cytoplasm is regulated by the CCHC-type zinc finger. Upon UV-C stress stimulus, the nuclear concentration of the protein decreases, affecting alternative splicing. Translocates from the nucleus to the cytoplasm after heat shock cell treatment. Accumulates in cytoplasmic vesicle-like organelles after heat shock treatment, which may represent stress granules|
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Provided below are standard protocols that you may find useful for product applications.
Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by this gene is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions. [provided by RefSeq].
Alberstein, M., et al. RNA 13(11):1988-1999(2007)
Olsen, J.V., et al. Cell 127(3):635-648(2006)
Shomron, N., et al. J. Cell. Sci. 118 (PT 6), 1151-1159 (2005) :
Shomron, N., et al. Mol. Biol. Cell 15(8):3782-3795(2004)
Chua, K., et al. Mol. Cell. Biol. 21(5):1509-1514(2001)
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