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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   SHARPIN Antibody (Center)   

SHARPIN Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - SHARPIN Antibody (Center) AP18513c
    SHARPIN Antibody (Center) (Cat. #AP18513c) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the SHARPIN antibody detected the SHARPIN protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9H0F6
Other Accession NP_112236.3
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 39949 Da
Antigen Region 240-269 aa
Additional Information
Gene ID 81858
Other Names Sharpin, Shank-associated RH domain-interacting protein, Shank-interacting protein-like 1, hSIPL1, SHARPIN, SIPL1
Target/Specificity This SHARPIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-269 amino acids from the Central region of human SHARPIN.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSHARPIN Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms SIPL1
Function Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis.
Cellular Location Cytoplasm, cytosol. Cell junction, synapse. Note=Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1.
Tissue Location Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors
Research Areas
Citations (0)

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SHARPIN may have a role in normal immune development and control of inflammation (By similarity).


Jung, J., et al. Mol. Cell. Biochem. 340 (1-2), 161-167 (2010) :
Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
Daigo, Y., et al. J. Gastroenterol. Hepatol. 18(6):712-718(2003)
Lim, S., et al. Mol. Cell. Neurosci. 17(2):385-397(2001)

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Cat# AP18513c
(40 western blots)
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