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PI3KC3 Antibody (C-term E785)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PI3KC3 Antibody (C-term E785) AP1851b
    Western blot analysis of lysate from U-251 MG cell line, using hPI3KC3 (C-term)(Cat. #AP1851b). AP1851b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
  • IHC-P - PI3KC3 Antibody (C-term E785) AP1851b
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with hPI3KC3 (C-term) (Cat.#AP1851b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8NEB9
Other Accession O88763, Q5D891, Q6PF93
Reactivity Human
Predicted Mouse, Pig, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 101549 Da
Antigen Region 770-801 aa
Additional Information
Gene ID 5289
Other Names Phosphatidylinositol 3-kinase catalytic subunit type 3, PI3-kinase type 3, PI3K type 3, PtdIns-3-kinase type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, hVps34, PIK3C3, VPS34
Target/Specificity This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 770-801 amino acids from the C-terminal region of human PI3KC3.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI3KC3 Antibody (C-term E785) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIK3C3
Synonyms VPS34
Function Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.
Cellular Location Midbody. Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Tissue Location Ubiquitously expressed, with a highest expression in skeletal muscle.
Research Areas
Citations ( 0 )


PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.


Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003).
Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).

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$ 295.00
$ 99.00
Cat# AP1851b
(40 western blots)
Availability: In Stock
Bulk Size
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